| Antibodies |
| anti-mouse CD1d (1B1) FITC |
BD Biosciences |
Cat# 553845 |
| anti-mouse a-GalCer:CD1d complex PE |
Biolegend |
Cat# 140505 |
| anti-mouse aryl hydrocarbon receptor PE |
BD Biosciences |
Cat# 5657711 |
| Chemicals, Peptides, and Recombinant Proteins |
| Dialyzed FBS |
GIBCO |
custom for R.S.B. Laboratory |
| Dialyzed RPMI |
GIBCO |
custom for R.S.B. Laboratory |
| Critical Commercial Assays |
| Mouse IL-13 DuoSet ELISA |
R&D Systems |
Cat# DY413 |
| Mouse IFN-γ ELISA MAX |
Biolegend |
Cat# 430801 |
| Mouse IL-10 ELISA Ready-Set-Go |
eBioscience |
Cat# 501125188 |
| IDO1 Inhibitor Screening Assay Kit |
BPS Biosciences |
Cat# 72021 |
| Luciferase Assay System |
Promega |
Cat# E4550 |
| Lipofectamine 2000 |
Thermo Fisher Scientific |
Cat# 11668019 |
| Intracellular Fixation and Permeablization Buffer Set |
eBioscience |
Cat# 88882400 |
| Omniscript RT Kit |
QIAGEN |
Cat# 205111 |
| SYBR Green I |
Roche |
Cat# 3003230001 |
| Oligonucleotides |
| 5′-GATGCTCCCCGGGCTGTATT-3′ |
Mouse Bactin qPCR Forward Primer |
R.S.B. Laboratory |
| 5′-GGGGTA CTTCAGGGTCAGGA-3′ |
Mouse Bactin qPCR Reverse Primer |
R.S.B. Laboratory |
| 5′-GAGAGCTGCAGGGCCCTTTGC-3′ |
Mouse Interleukin 10 qPCR Forward Primer |
R.S.B. Laboratory |
| 5′-CTCCCTGGTTTCTCTTCCCAAGACC-3′ |
Mouse Interleukin 10 qPCR Reverse Primer |
R.S.B. Laboratory |
| 5′-GCAGCCAGTACGCTCTTTTC-3′ |
Mouse CD1d qPCR Forward Primer |
R.S.B. Laboratory |
| 5′-ACAGCTTGTTTCTGGC AGGT-3′ |
Mouse CD1d qPCR Reverse Primer |
R.S.B. Laboratory |
| ′-GGACTTTTTGGATTTCAAAAGTGAC-3′ |
Mouse Mttp qPCR Forward Primer |
R.S.B. Laboratory |
| 5′-GGAGAAACGGTCATAATTGTG-3′ |
Mouse Mttp qPCR Reverse Primer |
R.S.B. Laboratory |
| 5′-TGGCACTCAGTAAAATATCTCCT-3′ |
Mouse IDO1 qPCR Forward Primer |
R.S.B. Laboratory |
| 5′CAGGCAGATTTCTAGCCACA-3′ |
Mouse IDO1 qPCR Reverse Primer |
R.S.B. Laboratory |
| 5′-GGCCACTTTGACCCTTACAA-3′ |
Mouse Cyp1a1 qPCR Forward Primer |
R.S.B. Laboratory |
| 5′-CAGGTAACGGAGGACAGGAA-3′ |
Mouse Cyp1a1 qPCR Reverse Primer |
R.S.B. Laboratory |
| mouse AhR siRNA |
Thermo Fisher Scientific |
Cat# 100360 |
| control siRNA |
Thermo Fisher Scientific |
Cat# AM4611 |
| Experimental Models: Cell Lines |
| MODE-K small intestinal epithelial cell line |
Vidal et al., 1993 |
R.S.B. Laboratory |
| 24.7 invariant Natural Killer T cell hybridoma |
N/A |
R.S.B. Laboratory |
| 24.8 invariant Natural Killer T cell hybridoma |
N/A |
R.S.B. Laboratory |
| 14S.6 non-invariant Naturall Killer T cell hybridoma |
N/A |
R.S.B. Laboratory |
| Experimental Models: Organisms/Strains |
| C57BL/6J Specific Pathogen Free |
The Jackson Laboratory |
Cat# 000664 |
| CD1d 3/3 |
N/A |
R.S.B. Laboratory |
| Ahr tm3.1Bra/J |
The Jackson Laboratory |
Cat# 006203 |
| Villin-Cre |
N/A |
R.S.B. Laboratory |
| C57BL/6 Germ Free |
Harvard Digestive Disease Center |
R.S.B. Laboratory |
| AhR 3/3 |
Taconic |
Cat# 9166 |
| C57BL/6 |
Taconic |
Cat# B6 |
| Recombinant DNA |
| pGL4.43 Luc2pXRE |
Promega |
Cat# E4121 |
| pGL4.75 |
Promega |
Cat# E6931 |
| Renilla luciferase |
Promega |
Cat# E2261 |
| Software and Algorithms |
| Graphpad Prism 7.0 |
GraphPad Sofware |
https://www.graphpad.com/ |
| FloJo |
FloJo, LLC and Illumina, Inc |
https://www.flowjo.com/ |
| Other |
| Nikon Eclipse Ti Microscope |
Nikon |
N/A |
| BD FACS Aria II |
BD Biosciences |
N/A |
| MACSQuant Analyzer |
Miltenyi Biotec |
N/A |
| LTQ-XL Linear Ion Trap Mass Spectrometer |
Thermo Fisher Scientific |
N/A |
| CFX96 Real-Time System |
Bio-Rad |
N/A |
| 4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one |
Sigma |
Cat# 862207 |
| 2,4,5-trimethyl-2,5-dihydro-1,3-oxazole |
Sigma |
Cat# CDS001470 |
| 2-methyl-1-pyrroline |
Sigma |
381055 |
| Frag-oz |
Collin et al., 2013 |
A.L Laboratory |
| Frag-tz |
Collin et al., 2013 |
A.L. Laboratory |
| frag-oztz |
Collin et al., 2013 |
A.M. Laboratory |
| frag-tzoz |
Collin et al., 2013 |
A.M. Laboratory |
| Microcin B17 |
Collin et al., 2013 |
A.M. Laboratory |
| Vinclozolin |
Sigma |
Cat# 45705 |
| L-Tryptophan |
Sigma |
Cat# T0254 |
| DL-methyl tryptophan |
Sigma |
Cat# 860646 |
| L-trytophan-D5 |
Sigma |
Cat# sc-391262A |
| ITE |
Tocris |
Cat# 1803 |
| Subtilisin |
Sigma |
Cat# P5380 |
| Kynurenic-3,5,6,7,8-d5 |
CDN Isotopes |
Cat# D-4391 |
| L-Kynurenine |
Sigma |
Cat# K8625 |
| Kynurenic Acid |
Sigma |
Cat# K3375 |
| 4,8-dihydroxyquinoline-2-carboxylic acid |
Sigma |
Cat# D120804 |
| KRN7000 |
Avanti Polar Lipids, Inc |
Cat# 867000 |
| beta-glucosyl ceramide |
Avanti Polar Lipids, Inc |
Cat# 860547P |
| 1,2,4-trimethyl cyclopentane |
Sigma |
Cat# R289728 |
