FIGURE 1.

Ezrin phosphorylation in target cells is required for efficient X4-tropic HIV-1 infection. (A) Expression levels of C-terminally VSV-G epitope-tagged EZ-Wt, EZ-TA, and EZ-TD proteins in HeLa/CD4 cells were analyzed by western immunoblotting using the anti-VSV-G epitope antibody (upper panel). Actin protein served as a control (lower panel). (B) Transduction titers of the X4-tropic Env- and VSV-G-pseudotyped HIV-1 vectors were measured for the empty vector-, EZ-Wt-, EZ-TA-, and EZ-TD-expressing HeLa/CD4 cells. Transduction titers of the empty vector-expressing cells were always set to 1, and relative values of transduction titers in other cells are indicated. Error bars show standard deviations, and asterisks indicate statistically significant differences compared to the empty vector-expression cells.