Figure 2.

Schematic depicting the fabrication process of injectable HA-co-Gelatin cryogels. HAGM and/or MA-gelatin are dissolved in water containing an initiator system (APS and TEMED), and subsequently frozen at three different subzero temperatures (−20, −50 and −80 °C). The cryopolymerization and gelation of polymers occur around ice crystals, leaving interconnected voids behind upon melting. Rhodamine-labeled HAGM or MA-gelatin were incorporated only for microscopy imaging. Fabricated macroporous cryogels (4 mm × 4 mm × 1 mm) can then be easily and successfully syringe-injected through a 16-gauge hypodermic needle.