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(A) Total amount time spent in the center (TCA); (B) Number of return into center area of the arena in the open-field behavior apparatus (NRC); and (C) Number of total squares (NTS) in the open field in female and male rats after 2 month of treatment with 0.9% of NaCl (Control), 0.25 mg/kg (Ni-0.25), 0.5 mg/kg (Ni-0.5), and 1 mg/Kg (Ni-1) of Ni. Results are expressed as mean ± SEM. The significance level is 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001.
