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Attached nisin content (Bradford assay from electrophoresis) and nitrogen content (TN) on the PVA surface (PVA films were stored at 25 °C for 3 months, relative humidity 54%). Nisin content on untreated PVA films was not measurable, and SD was higher than calculated value.
| PVA/Nisin-Buffer Crosslinking Degree (%) | Nisin Content (µg/mm2) | TN (µg/mm2) |
|---|---|---|
| 0 | 1.8 ± 0.5 | 0.7 |
| 5 | 3.6 ± 1.5 | 4.3 |
| 10 | 31.4 ± 1.5 | 13.2 |
| 20 | 28.2 ± 1.5 | 9.7 |
| 40 | 19.3 ± 1.3 | 6.8 |
