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. 2018 Aug 27;9:1177. doi: 10.3389/fpls.2018.01177

FIGURE 4.

FIGURE 4

Interaction of ARPF2 with ARRS1 in the nucleolus. (A) Nucleolar localization of GFP fused to ARPF2 (ARPF2-GFP) and ARRS1 (ARRS1-GFP). Fluorescence from GFP and FIB1-mCherry (a nucleolar marker) was simultaneously monitored in Nicotiana benthamiana cells transiently expressing these proteins. Images for GFP were merged with those for fluorescence from FIB1-mCherry (Merged). DAPI staining indicates the nucleoplasm. Scale bars: 10 μm. (B) BiFC analyses for ARPF2 and ARRS1. ARPF2 fused to nGFP (ARPF2-nGFP) was co-expressed with cGFP-fused ARRS1 (ARRS1-cGFP) in N. benthamiana cells. The expression of cGFP and nGFP fused to the respective proteins alone served as negative controls. Scale bars: 10 μm. (C) Co-immunoprecipitation of ARPF2 and ARRS1. ARRS1-MYC was transiently expressed with or without ARPF2-GFP in N. benthamiana cells. The ARPF2-GFP protein in the crude cell extract (Input) was immunoprecipitated with an anti-GFP antibody, and the obtained precipitates (Output) were analyzed with anti-GFP and anti-MYC antibodies.