Fig. 8.

Effects of U0126 on cell viability in SAECs stimulated with H₂O₂. Cell viability was assessed by measuring formazan production from viable cells (at 450 nm) as described in the Methods. Samples were obtained from control cells, from cells pretreated with 10 μM U0126 alone for 0.5 h, or from cells pretreated with or without 10 μM U0126 for 0.5 h before stimulation with 100 μM H₂O₂ for 3 h. Data are presented as means ± SD of three independent experiments. ^**p < 0.01 vs. control cells, ^##p < 0.01 vs. cells pretreated with 10 μM U0126 alone, ^††p < 0.01 vs. cells pretreated without 10 μM U0126 before H₂O₂ stimulation (cells stimulated with H₂O₂ alone)