Table 1.

Detailed MS/MS parameters

substance	Q1 (m/z)	Q3 (m/z)	Declustering potential (V)	Collision energy (V)	Cell exit potential (V)
Phloretin	273	167/123	−20	−25	−6
Phloretin glucuronide	449	273/123	−152	−20	−7
Procyanidin B1	577	289/407	−35	−18	−12
Procyanidin B2	577	407/289	−35	−33	−12
Procyanidin B5	577	425/289	−35	−18	−12
Procyanidin B7	577	425/289	−35	−18	−12
(Epi)catechin	289	245/109	−45	−21	−7
Quercetin	301	179/273	−30	−25	−8

Multiple reaction monitoring transitions were tuned on an AB Sciex Qtrap 5500® mass spectrometer with direct infusion of neat standard solutions (or diluted urine samples for phloretin glucuronide). The declustering potential, entrance potential, collision energy, and collision cell exit potential were optimized for each multiple reaction monitoring transition. The two most intense multiple reaction monitoring transitions were used as quantifier/qualifier to build the final method.