Figure 8.

Gα_s enhances signaling when Hh levels are low. All data represent mean ± S.D. t test: **, p < 0.01; ***, p < 0.001; n.s., not significant. A, ptc-luc reporter assay of Smo^WT-GFP-expressing cells co-transfected with increasing amounts of Hh^N expression plasmid (in ng). B, ptc-luc reporter assay of cells transfected with empty vector (−) or with Smo^WT-GFP and 100 ng of a Hh^N expression plasmid, and treated with dsRNAs targeting β-gal (control) or Gαs. C, EPAC-BRET assay of cAMP levels in cells co-transfected with Smo^WT-GFP and either empty vector (0) or 20 or 100 ng of Hh^N expression plasmid. D, ptc-luc reporter assay of cells transfected with empty vector (−) or with Smo^WT-GFP and 20 ng of a Hh^N expression plasmid, and treated with dsRNAs targeting β-gal (control) or Gαs.