Figure 1.

Reduction of BM nucleated cells and progenitor subpopulations in Fpr2^−/−mice. A and B, reduction of the total number of BM nucleated cells in Fpr2^−/− mice. BMCs from the femora of male mice at the age of 3 or 6 weeks (WK) were obtained and counted. A, the mean number of BMCs from two femora of each male mouse at the age of 3 weeks. n = 11–13 mice/group. *, p < 0.05. B, the number of BMCs from two femora of each male mouse at the age of 6 weeks. n = 9–10 mice/group. *, p < 0.05. C and D, reduction of BM progenitor cells in Fpr2^−/− mice. BMCs from male mice at the age of 3 weeks were preincubated in FACS buffer with anti-mouse CD16/32 mAb for 20 min at 4 °C to eliminate nonspecific Ab binding to FcγII/IIIR. The cells were then stained with anti-mouse Ly6C-PE and CD31-FITC Abs and analyzed by FACS. C, two-color FACS analysis of CD31 and Ly6C expression by total BM cells. D, six phenotypically distinct subpopulations in the BM of WT and Fpr2^−/− mice. *, p < 0.05, significantly reduced cell number in the BM of Fpr2^−/− mice. n = 4 mice/group with three independent experiments.