Figure 6.

The phosphorylation of P38, STAT1, Akt (Thr-308) and Akt (Ser-473) induced by SCF in c-Kit⁺ cells. A, SCF-induced phosphorylation. BM nucleated cells were stimulated with SCF (10 ng/ml) for 1 h and then stained with anti-mouse c-Kit-FITC Ab and anti-mouse phosphorylated p-p38, p-Akt (Thr-308), p-Akt (Ser-473), and p-STAT1 Abs as well as matched IgG as a control. The cells were analyzed by FACS. B, cumulative results for the phosphorylation of p38, STAT1, Akt (Thr-308), and Akt (Ser-473) induced by SCF. Results are expressed as the mean ± S.E. n = 3 mice/group, and the experiments were performed three times. *, p < 0.05. FSC-H, forward-scattered light–height.