Figure 8.

Effect of Cav-1 (Y14F) mutation on the interaction between Cav-1 and Oct4. A, H460 cells were transfected with Cav1-(Y14F)–Myc–His or FLAG–Cav1. The transfected cells were subjected to co-immunoprecipitation by pulling down the tagged proteins (IP:His for Cav1(Y14F)Myc-His and IP:FLAG for FLAG-Cav1) and measuring the level of Oct4 by Western blotting. B, 3D model of WT Cav-1 (wtCav1) (green) bound with Oct4 (purple), where the Tyr-14 residue is presented as a green stick. C, close-up of the Cav-1 residue 14 on intermolecular hydrogen bond interaction with the Oct4 residues (Gly-308) (dashed line) for the WT, pTyr-14, and Y14F Cav-1:Oct4 complexes.