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. 2018 Jul 10;293(35):13351–13363. doi: 10.1074/jbc.RA118.004324

Figure 4.

Figure 4.

Dissociation rate constants (koff) and strand transfer efficiencies of WT BH10 and 3M_T355A/Q357M RTs with primers and acceptors of different lengths. A, dissociation rate constants (koff) determined with RNA/DNA hybrids containing primers of 23–27 nucleotides complexed with the RNA template donor R33B. Reactions were carried out at 37 °C using template-primers R33B/23A, R33B/24A, R33B/25A, R33B/26A, and R33B/27A (10 nm) and an RT concentration of 30 nm. B, effect of the overlapping sequence size on the strand transfer efficiency of WT BH10 and 3M_T355A/Q357M RTs. Strand transfer reactions were carried out with the [32P]DNA/RNA primer–donor complex 20A/R33B, in the presence of DNA acceptors of variable lengths. The used acceptors differ from each other in the number of overlapping nucleotides with the 5′ end of the donor R33B (see Fig. 1 for details). Reported strand transfer efficiencies were obtained after incubating the sample for 60 min and were the averages of three independent determinations.