Figure 8.
Molecular model of HIV-1ESP49 RT in a ternary complex with dsDNA and dTTP, showing the location of substituted amino acids in the studied mutants. The model was built by using the structure of WT HIV-1 RT (HXB2) strain bound to dsDNA and dTTP as template (29). RT subunits are represented by cyan and green ribbons. The incoming dTTP is shown in orange; the side chains of Phe-61, Leu-92, and Val-148 are shown in purple, and the side chains of connection subdomain residues 358–360 are shown in dark gray. The template and primer strands are depicted in red and yellow, respectively.