Table 1.
Dissociation rate constants (koff) of WT and mutant HIV-1 RTs
Reported constants were obtained with the RNA/DNA template-primer R33B/20A and represent the average ± S.D. of at least three independent experiments. Numbers in parentheses represent the fold-increase of the koff relative to the value obtained with WT BH10 RT.
| RTs | koff |
|---|---|
| s−1 | |
| WT BH10 | 0.0141 ± 0.0010 |
| BH10_L92P | 0.0684 ± 0.0130 (4.8) |
| WT ESP49 | 0.0240 ± 0.0034 (1.7) |
| 3M | 0.0203 ± 0.0021 (1.4) |
| 3M_L92P | 0.0913 ± 0.0100 (6.5) |
| 3M_D443N | 0.0051 ± 0.0005 (0.4) |
| 3M_L92P/D443N | 0.0127 ± 0.0014 (0.9) |
| 3M_E478Q | 0.0054 ± 0.0006 (0.4) |
| 3M_L92P/E478Q | 0.0160 ± 0.0018 (1.1) |
| 3M_F61A | 0.0212 ± 0.0020 (1.5) |
| 3M_K65R/E478Q | 0.0057 ± 0.0004 (0.4) |
| 3M_V148I | 0.0288 ± 0.0053 (2.0) |
| 3M_T355A/Q357M | 0.0241 ± 0.0020 (1.7) |