Figure 2.
Gentamicin protection assay measuring VHH-mediated inhibition of Listeria invasion. HeLa cells were infected with L. monocytogenes in the presence of PBS buffer control, InlB (positive control to inhibit Listeria invasion), irrelevant VHH negative control (20), and anti-InlB VHH R303, R326, R330, and R419. Invasion was quantified by counting the number of intracellular L. monocytogenes released from infected HeLa cells following gentamicin treatment. The percentage inhibition was calculated relative to the PBS buffer control. VHH R303 and R330 inhibited L. monocytogenes similar to the InlB control treatment, R326 was modestly effective at inhibiting invasion, and R419 was not effective. The center horizontal bars represent the mean, and error bars represent the S.D. (n = 3).