Figure 6.

Structural illumination microscopy of sfGFP-fused VirB6a and its variants. A, C-terminal fusions of VirB6 and of variants VirB6aL18A, VirB6aF20A, VirB6aΔ(18–23), and VirB6aΔ(30–35) to sfGFP were expressed in strain CB1006 and imaged by SIM. B, quantification of the fluorescence using the CTCF method, showing preferentially polar localization for each sample (****, p < 0.0001). Values and standard deviations were calculated from three independent experiments. The p values were obtained by t test, for each construction, between the overall bacteria and pole 1 or pole 2. ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001.