Figure 7.

TAM‐like THP1 stimulate proliferation and reduce chemosensitivity of MDA468 cells. The cocultures of GFP‐Luc‐labeled MDA468 cells and Ctr THP1 or TAM‐like THP1 were prepared as illustrated in Fig. 6. (A) Cancer cell confluence/proliferation was tracked over time by IncuCyte ZOOM, recording the GFP signal. The confluence at 24 h postseeding was set to 1, and the increase in the confluence over time is indicated; average ± SEM (n = 5); *P < 0.05 for 48–72 h. (B) Both cocultures were treated with carboplatin or paclitaxel at the indicated concentrations for 3 days. The proliferation/viability of the treated cancer cells was scored by measuring Luc‐mediated bioluminescence and is presented as % from respective untreated controls (average ± SEM, n = 3–4).