Table 1.

Primers and siRNA sequence

Primer and siRNA	Sequence
GAPDH forward	5′‐CGGATTTGGTCGTATTGGG‐3′
GAPDH reverse	5′‐CTGGAAGATGGTGATGGGATT‐3′
DR6 forward	5′‐ATCCGGAAAAGCTCGAGGAC‐3′
DR6 reverse	5′‐CTTCCCACTTGGGCTGCTAC‐3′
KIF11 forward	5′‐GCCCCAAATGTGAAAGCATT‐3′
KIF11 reverse	5′‐CTAAAGTGGGCTTTTTGTGAACTCT‐3′
TRAF4 forward	5′‐CCACCGTTTCTGCGATACCT‐3′
TRAF4 reverse	5′‐GTCTGGGTAGATCTTGGCATAG‐3′
shDR6‐1 forward	5′‐GCAGCCTATCTTTGATGACAT‐3′
shDR6‐1 reverse	5′‐ATGTCATCAAAGATAGGCTGC‐3′
shDR6‐2 forward	5′‐CCAACGCGAAACTTGAGAATT‐3′
shDR6‐2 reverse	5′‐AATTCTCAAGTTTCGCGTTGG‐3′
DR6 signal peptide forward	5′‐CTAGCTAGCGCCACCATGGGGACCTCTCCGAGCAG‐3′
DR6 signal peptide reverse	5′‐AGCTGTGGTGGTGCTAAGGAATCCAAGCAGGAGAA‐3′
3×flag forward	5′‐GACTACAAAGACCATGACGGTGATTA‐3′
3×flag reverse	5′‐CTTGTCATCGTCATCCTTGTAGTCGATGTC‐3′
DR6 (without signal peptide) forward	5′‐CAGCCAGAACAGAAGGCCTCGAATCTCATTGGCAC‐3′
DR6 (without signal peptide) reverse	5′‐GCTCTAGATTACTACAGCAGGTCAGGAAG‐3′
Fusion sequence (DR6 signal peptide and 3×flag) forward	5′‐CTAGCTAGCGCCACCATGGGGACCTCTCCGAGCAG‐3′
	5′‐TCCTTAGCACCACCACAGCTGACTACAAAGACCATGAC‐3′
Fusion sequence (DR6 signal peptide and 3×flag) reverse	5′‐CCGTCATGGTCTTTGTAGTCAGCTGTGGTGGTGCTAAG‐3′
	5′‐CTTGTCATCGTCATCCTTGTAGTCGATGTCATGA‐3′
Fusion sequence (DR6 signal peptide and 3×flag and DR6) forward	5′‐CTAGCTAGCGCCACCATGGGGACCTCTCCGAGCAG‐3′
	5′‐ACAAGGATGACGATGACAAGCAGCCAGAACAGAAGGCC‐3′
Fusion sequence (DR6 signal peptide and 3×flag and DR6) reverse	5′‐GAGGCCTTCTGTTCTGGCTGCTTGTCATCGTCATCCTTG‐3′
	5′‐GCTCTAGATTACTACAGCAGGTCAGGAAGA‐3′
KIF11 siRNA sense	5′‐UCGAGAAUCUAAACUAACU‐3′
KIF11 siRNA antisense	5′‐AGUUAGUUUAGAUUCUCGA‐3′
TRAF4 siRNA sense	5′‐GCACTAAGGAGTTCGTCTT‐3′
TRAF4 siRNA antisense	5′‐AAGACGAACUCCUUAGUGC‐3′

GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; KIF11, kinesin family member 11; shDR6‐1, short hairpin DR6‐1; shDR6‐2, short hairpin DR6‐2; TRAF4, TNF receptor‐associated factor 4.