Figure 7.

Effect of miR‐9 on EV71 replication and pathogenicity in mice. Mice were inoculated with EV71 or PBS as a negative control. Mice were then treated with miR‐9 agomir. (A) Mice from the EV71 group and the NC group were sacrificed on day 4 or 5 postinoculation to determine miR‐9 expression in different tissues of mice. Expression levels of VP1 (B), IL‐1 (C), and IFN‐β (D) in different tissues of EV71‐infected mice were examined using real‐time PCR. These assays have been performed three times. Data represent means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. The statistical test performed using Student's t‐test.