Figure 2.

Adipogenic differentiation of orbital and abdominal ASCs towards adipogenesis. (a) Microscopic photograph of ASCs cultured in adipogenic medium for 21 days and subjected to lipid staining with Oil Red O. Control cells were grown in DMEM/F12 medium for the same period of time. (b) Quantitation analysis of lipid droplet accumulation by determining the amount of dye extracted with isopropanol and measured at absorbance 540 nm. (c) Real-time PCR analysis for genes involved in adipogenesis, such as PPARγ, C/EBPα, and FABP4. Results are presented as means ± standard error mean (SEM). ^∗p < 0.05 and ^∗∗p < 0.01.