Table 1.
Primer Name | Sequence (5'-3') | Product size (bp) | TM (°C) |
---|---|---|---|
KIT_E1F | GGAACGTGGAAAGGAGCT | 205 | 66 |
KIT_E1R | CAGCCACCTCTTCTGACAC | ||
KIT_E2F | ACCCTTGCACCATAAATAGC | 498 | 59 |
KIT_E2R | GACTCAGCCATCTATATGTC | ||
KIT_E3F | TCAGATTCGTTAACCACTGC | 447 | 64 |
KIT_E3R | TGGCATATCCGCTGATCTA | ||
KIT_E4F | CGAAAGGCCATACAGTGAG | 362 | 50 |
KIT_E4R | TGTATGAAAGCAACTATACC | ||
KIT_E5F | TCCCCCCGGGACTGCAGAGATTTGGGAATTATG | 548 | 59 |
KIT_E5R | CGCGGATCCTCAACCTACTACTTCTAAGGTGG | ||
KIT_E6F | GAAGACATAAGATGGTGATA | 385 | 53 |
KIT_E6R | CTAATATCCATGAGGACGCAGG | ||
KIT_E7F | CCAAAGCAGGCGTGTATTT | 344 | 55 |
KIT_E7R | GGTCAAAATTATCATGAAGCAG | ||
KIT_E8F | GTCATCTTCAGCCTCAAGAAAG | 313 | 54 |
KIT_E8R | TCTGAAAACATATTTGAAATTC | ||
KIT_E9F | GGCAAATAATTTTTCTTCTAG | 293 | 54 |
KIT_E9R | CAGGCAGAGCCTAAACATCC | ||
KIT_E10-11F | CTTGACTCCTGCCATGATG | 536 | 64 |
KIT_E10-11R | CTAGAACAAAGGAAGCTACCG | ||
KIT_E12-13F | GTCCCTGATTCCTTTATTGG | 486 | 60 |
KIT_E12-13R | GCTTATCATCAAGGATGGTC | ||
KIT_E14F | GCATGTGAATGGCCGTGATC | 322 | 50 |
KIT_E14R | ATCAGCCTTGATTGCAAACC | ||
KIT_E15-16F | CAAAGTCAGCCTCTTATGTGA | 910 | 52 |
KIT_E15-16R | CTACGGCTCTAAAATGCTCC | ||
KIT_E17F | TGGCACCATATAACATAGGC | 325 | 60 |
KIT_E17R | GGTGTGCATTATGAAACTCAC | ||
KIT_E18-19F | GCAGCAGGAGCAGTATCTAC | 482 | 63 |
KIT_E18-19R | AACCTTCAACATCTGGGTTC | ||
KIT_E20F | GGTGTACACCCAAAGACAG | 217 | 63 |
KIT_E20R | CTGTTCAAGGCGTTCCAAGC | ||
KIT_E21F | ACTTGCGATTCTGGACCTGC | 271 | 63 |
KIT_E21R | GAATGGCAGTAGGTCGGTGC |