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. Author manuscript; available in PMC: 2018 Dec 13.
Published in final edited form as: Nature. 2018 Jun 13;558(7710):401–405. doi: 10.1038/s41586-018-0211-2

Extended Data Figure 2. CryoEM and 3D Reconstruction.

Extended Data Figure 2

(a) An electron cryo-micrograph of DRP1-MID49126-454 filaments formed with GMPPCP. Bar =100nm. Inset shows a representative 2D class average. Inset bar =10nm.

(b) Cross-section of the 3D reconstruction of the filament and the distribution of views determined during helical reconstruction. The length of the cylinders and the color code correspond with the number of particles for that viewing direction (from few to many, blue to red). The 3D structure has been segmented and colored with DRP1 in grey and MID49 in golden yellow.

(c) Particle numbers and workflow for the reconstruction protocol. DRP1 density is shown in grey and MID49 is in golden yellow.

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