Table 1.

Number of acantharian symbionts visible by microscopy and symbiotic SVs observed per host.

	Symbionts visible in micrograph	Symbiotic SVs per host
Oki.3A	20	6
Oki.4A	17	10
Oki.3	29	10
Oki.7	38	8
Oki.10	28	12
Oki.11	24	5

Symbionts within 6 acantharians collected in April (Oki.3A and Oki.4A) and May (Oki.3, Oki.7, Oki.10, Oki.11) from coastal waters near Okinawa, Japan were enumerated by visualizing chlorophyll autofluorescence with laser confocal microscopy. More symbionts were observed in each of the imaged hosts than were detected from analyzing amplicon sequence data.