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. 2018 Jun 25;182(4):567–578. doi: 10.1111/bjh.15441

Figure 5.

Figure 5

Effect of hypochlorous acid (HOCl) on phosphatidylserine (PS) exposure of red cells from sickle cell anaemia patients. (A) Red cells were pre‐loaded with CM‐H2DCF‐DA (100 μmol/l) or treated with the same final [DMSO] before incubation with HOCl (500 μmol/l) at 0·5% haematocrit for 30 min at 37°C in HK‐HBS (n = 3). (B) Red cells were permeabilised to Ca2+ as in Fig 1B at 0·5% haematocrit for 30 min at 37°C in the absence (full circles) or presence (open circles) of HOCl (500 μmol/l) after which accessible PS was labelled with LA‐FITC (n = 6). PS exposure was normalised to that of control red cells at 1 μmol/l free [Ca2+]o (32 ± 5·4% of total red cells). Symbols represent means ± SEM for red cells from n different individuals.