Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 25;182(4):567–578. doi: 10.1111/bjh.15441

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors. British Journal of Haematology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Schematic diagram of the effect of tert‐butyl hydroperoxide (tBHP) on red cells from patients with sickle cell anaemia. (i) tBHP is thought to increase phosphatidylserine (PS) exposure but relatively high concentrations have been used, which may disrupt the integrity of the membrane; (ii) in the presence of high tBHP more PS was labelled, but the ability of anti‐Hb IgG to label intracellular Hb suggests that this was because loss of membrane integrity allowed labelling of both inner leaflet PS as well as externalised PS; (iii) by contrast, with lower tBHP concentrations that do not allow entry of anti‐Hb IgG, PS exposure was reduced by 50%, as for the other oxidants (in Fig 1b). ●, known PS distribution; , PS distribution unknown.

Inline graphic — Schematic diagram of the effect of tert‐butyl hydroperoxide (tBHP) on red cells from patients with sickle cell anaemia. (i) tBHP is thought to increase phosphatidylserine (PS) exposure but relatively high concentrations have been used, which may disrupt the integrity of the membrane; (ii) in the presence of high tBHP more PS was labelled, but the ability of anti‐Hb IgG to label intracellular Hb suggests that this was because loss of membrane integrity allowed labelling of both inner leaflet PS as well as externalised PS; (iii) by contrast, with lower tBHP concentrations that do not allow entry of anti‐Hb IgG, PS exposure was reduced by 50%, as for the other oxidants (in Fig 1b). ●, known PS distribution; , PS distribution unknown.