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. 2018 Aug 27;9:1194. doi: 10.3389/fpls.2018.01194

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Copyright © 2018 Inácio, Martins, Graça and Morais-Cecílio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of 5-methylcytosine (5-mC) in lenticels from three-year-old sprigs. Less intense 5-mC signals in lenticular cells compared with the contiguous tissues like lenticular phellogen, phellogen, cork cells, and cortex (A). (B) Fragmented nuclei of lenticular cells showing 5-mC signals. (C) Lenticular phellogen exhibit significant lower levels of 5-mC than the contiguous ‘cork’ phellogen; different small letters indicate significant differences. DNA was counterstained with DAPI. Bars = 10 μm. Lph, lenticular phellogen; Ph, phellogen; Lc, lenticular cells; C, cork cells; Co, cortex.