Table 4.
Calibration curve for the MUC5AC ELISA kit
| Day 1
|
Day 2
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample (pg/mL) | OD value | Mean OD value | Calculated concentration (pg/mL) | RE (%) | Sample (pg/mL) | OD value | Mean OD value | Calculated concentration (pg/mL) | RE (%) |
| 1,250 | 2.6967 | 2.69245 | 1,246.3 | −0.3 | 1,250 | 3.0226 | 3.04515 | 1,241.6 | −0.7 |
| 2.6882 | 3.0677 | ||||||||
| 625 | 1.4581 | 1.47225 | 635.5 | 1.7 | 625 | 1.8444 | 1.84630 | 652.8 | 4.4 |
| 1.4864 | 1.8482 | ||||||||
| 312.5 | 0.7581 | 0.81935 | 312.3 | −0.1 | 312.5 | 1.0084 | 1.02435 | 289.3 | −7.4 |
| 0.8806 | 1.0403 | ||||||||
| 156.3 | 0.4701 | 0.47225 | 142.5 | −8.8 | 156.3 | 0.6740 | 0.67590 | 151.6 | −3.0 |
| 0.4744 | 0.6778 | ||||||||
| 78.1 | 0.3292 | 0.32575 | 71.8 | −8.1 | 78.1 | 0.4133 | 0.44565 | 69.9 | −10.5 |
| 0.3223 | 0.4780 | ||||||||
| 39.1 | 0.2816 | 0.26790 | 44.2 | 13.0 | 39.1 | 0.3502 | 0.33755 | 36.0 | −8.0 |
| 0.2542 | 0.3249 | ||||||||
| 19.5 | 0.2240 | 0.22385 | 23.4 | 20.2 | 19.5 | 0.2948 | 0.30740 | 27.4 | 40.3 |
| 0.2237 | 0.3200 | ||||||||
| 9.8 | 0.1856 | 0.17970 | – | – | 9.8 | 0.2625 | 0.28725 | – | – |
| 0.1738 | 0.3120 | ||||||||
| 0 | 0.2072 | 0.20380 | – | – | 0 | 0.2283 | 0.23085 | – | – |
| 0.2004 | 0.2334 | ||||||||
Note: The relative error (RE) values were calculated by fitting the curve to a four-parameter model in the range of 39.1–1,250 pg/mL with 19.5 pg/mL set as the anchor point.