Figure 2.

HbF Reactivation Is Similar between Exon 2 and Enhancer KO

(A) Flow cytometry for HbF+ cells on days 14 and 20 of erythroid differentiation (note the higher levels in immature erythroid cells). (B) Immunocytochemistry in cytospins showing HbF+ cells in both coding and enhancer KO (day 20). (C) Enucleated HbF+ cells present in both edited populations. Blue, DAPI; green, anti-HbF (day 20). (D) Indel percentage of cell subpopulations sorted based on their size (small or large), reflecting maturation levels, and/or based on HbF expression (high or low) (day 20). (E) HPLC traces of unsorted, enucleated, and nucleated cells, showing increases in both γ-globin chains by exonic and enhancer KO (day 20).