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. 2018 Aug 14;10:313–326. doi: 10.1016/j.omtm.2018.08.003

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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HbF Reactivation in Ex Vivo Erythroid Cultures from Week 16 In Vivo Engrafted Human CD45+ Cells

(A) HbF+ cell frequency in enucleated and nucleated cells as evaluated by flow cytometry. (B) γ-globin/β-like globin and γ-globin/α-globin percentages as detected by HPLC. (C) Cell growth of erythroid cells. (D) Percentage of enucleation on day 20 of the erythroid culture. (E) Indel percentage of exon 2- and enhancer ZFN-treated cells on day 14 of the erythroid culture. *p < 0.05 versus untransfected (UNTR). The error bars represent the SEM of 4 mice.