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. 2018 Jul 23;37(17):e98984. doi: 10.15252/embj.201898984

Figure 1. The HSC number in the bone marrow achieved homeostasis at 4 weeks after birth.

Figure 1

  • A–C
    The bone marrow cellularity (A), CD150+CD48−Lineage−Sca‐1+c‐kit+ HSC frequency (B), and number (C) in the bone marrow of wild‐type mice at indicated weeks after birth. All data reflected mean ± SD of two tibias plus two femurs (n = 6 mice/age from three independent experiments). Two‐tailed Student's t‐tests were used to assess the statistical significance of differences between consecutive ages (*P < 0.05, **P < 0.01, ***P < 0.001).
  • D, E
    Competitive reconstitution assay in which 300,000 of donor‐derived bone marrow cells (D) or 0.5% of donor‐derived bone marrow cells (E) from 4‐ or 8‐week‐old mice were transplanted along with 500,000 recipient‐type competitor cells into irradiated recipient mice (n = 11–12 recipient mice/genotype from three independent experiments). Data represented mean ± SD. The statistical significance of differences was assessed using two‐way ANOVAs (***P < 0.001).
  • F–H
    Peripheral white blood cell count (WBC, F), red blood cell count (RBC, G), and platelet count (PLT, H) of wild‐type mice at indicated weeks after birth. All data reflect mean ± SD (n = 5 mice/time point from three independent experiments). Two‐tailed Student's t‐tests were used to assess the statistical significance of differences between consecutive ages (*P < 0.05, **P < 0.01, ***P < 0.001).