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. 2018 Jul 23;37(17):e98984. doi: 10.15252/embj.201898984

Figure 5. Lhcgr deletion did not affect HSC niche function.

Figure 5

  • A–D
    Flow cytometric analyses showed that Lhcgr deletion did not alter the frequency of CD45/Ter119−VE‐cadherin+ endothelial cells (A,B) or CD45/Ter119−PDGFRα+ perivascular cells (C,D) in the bone marrow. Panels (A) and (C) show the gating strategies for analyzing endothelial cells and perivascular cells, respectively, by flow cytometry. Panels (B) and (D) show the quantification results for endothelial cells and perivascular cells, respectively (n = 6 mice from three independent experiments). Data represented mean ± SD. Two‐tailed Student's t‐tests were used to assess the statistical significance between Lhcgr −/− and control mice.
  • E–H
    Confocal imaging of thick bone marrow sections (50 μm) showed no significant changes to the frequencies of CD41+ megakaryocytes (F), VE‐cadherinbrightLaminindim sinusoids (G) or VE‐cadherindimLamininbright arterioles (H). Representative confocal images are shown in (E) (n = 6 mice/genotype from three independent experiments). Data represented mean ± SD. Two‐tailed Student's t‐tests were used to assess the statistical significance between Lhcgr −/− and control mice.
  • I, J
    Quantitative real‐time PCR analyses of the transcript levels (normalized to β‐actin) of Scf (I) and Cxcl12 (J) in perivascular cells (Peri) and endothelial cells (Endo). Data represented mean ± SD (n = 3 mice from three independent experiments). Two‐tailed Student's t‐tests were used to assess the statistical significance between Lhcgr −/− and control mice.
  • K–M
    Bone marrow and spleen cellularity (L) and HSC numbers in the bone marrow and spleen (M) from 12‐week‐old Lhcgr −/− and control mice at 4 weeks after lethal irradiation and transplantation with one million bone marrow cells from wild‐type mice. Transplantation was performed as depicted in (K) (n = 6 mice/genotype from three independent experiments). Data represented mean ± SD. Two‐tailed Student's t‐tests were used to assess the statistical significance between Lhcgr −/− and control mice.