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. 2018 Jul 26;37(17):e99238. doi: 10.15252/embj.201899238

Figure 4. Mitochondrial inner membrane permeabilisation allows mtDNA release into the cytosol.

Figure 4

  1. Maximum intensity projection of Airyscan z‐stack data of U2OS cells treated with 10 μM ABT‐737, 1 μM ActD and 20 μM qVD‐OPh for 3 h. Cells were immunostained with anti‐AIF (IMM) and anti‐DNA antibodies. Z‐stack data were 3D‐rendered in Imaris and mtDNA nucleoid inside and outside the AIF signal were visualised. Scale bar = 5 μm. See [Link], [Link]. Representative images from three independent experiments.
  2. Airyscan images of U2OS cells stably expressing JF646‐MOM (magenta) treated with 10 μM ABT‐737, 1 μM ActD and 20 μM qVD‐OPh for 3 h and immunostained with anti‐DNA (red) and anti‐AIF (IMM, green) antibodies. Scale bar = 10 μm. Representative images from three independent experiments.
  3. Imaris 3D‐renderings of MOM (magenta), AIF (green) and DNA (red) from U2OS cells treated as in (B).
  4. U2OS cells stably expressing JF646‐MOM (magenta) and transiently expressing TFAM‐mScarlet (red) and AIF (1‐90)‐mClover (green) live‐cell imaged by Airyscan. Cells were treated with 10 μM ABT‐737, 2 μM S63845 and 20 μM qVD‐OPh at t = 0. Scale bar = 10 and 2 μm for zooms. See Video EV7. Representative images from two independent experiments. Numbers indicate time in minutes. Arrowheads indicate instances of released TFAM.