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. 2018 Jul 26;37(17):e99238. doi: 10.15252/embj.201899238

Figure EV4. mtDNA release is independent of mitochondrial fission and the mitochondrial permeability transition pore.

Figure EV4

  1. Expression of Drp1 protein in Wt and Drp1‐deleted MEFs.
  2. IncuCyte live‐imaging of SYTOX Green exclusion in Wt and Drp1‐deleted cells treated with 10 μM ABT‐737 and 1 μM ActD to assess cell viability. Data are expressed as mean ± SEM, representative of two independent experiments and have been normalised to starting confluency.
  3. Expression of CypD protein in Wt and CypD‐deleted MEFs.
  4. U2OS cells loaded with calcein‐AM and CoCl2 and imaged every 30 s for 20 min to show absence of photobleaching. Scale bar = 10 μm. Numbers indicate time in minutes.
  5. Images from time‐lapse live‐cell imaging of U2OS cells loaded with calcein‐AM and CoCl2 and treated with 10 μM ABT‐737, 2 μM S63845 and 20 μM qVD‐OPh in the presence of 25 μM CsA at t = 0. Scale bar = 10 μm. See Video EV9. Representative images from two independent experiments. Numbers indicate time in minutes.