Figure 3. Carbon metabolic flux tracing from isotopic labeled glucose in larval fat bodies.

• A
  Schematic of the experimental procedure for U‐¹³C glucose labeling of isolated Drosophila larval fat tissues.
• B, C
  Time‐course fractional ¹³C labeling of glycolytic metabolites and acetyl‐CoA (B) and TCA cycle metabolites (C) in the fat tissue after incubating with U‐¹³C‐glucose. The y‐axis in the graphs represents the ratio of intensities (area under the curve) of the labeled mass isotopomers to the sum of the labeled and unlabeled mass isotopomers for a given metabolite. n = 5 for each time point. Fat bodies from 15 larvae were used for each repeat. Error bars represent ±SEM.