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. 2018 Jul 26;37(17):e97572. doi: 10.15252/embj.201797572

Figure 4. External supplementation with citrate or pyruvate rescues the lipodystrophy of dSeipin mutants.

Figure 4

  1. 1% citrate or 1% pyruvate supplement rescues the fat body lipid storage phenotype of dSeipin mutants. BODIPY staining labels the lipid droplets and DAPI labels the nuclei. Scale bar represents 50 μm.
  2. Determination of TAG levels in third‐instar larval fat bodies of wild type or dSeipin mutants cultured on different foods, related to (A) (n = 6, fat bodies from ≥ 10 larvae were used for each repeat).
  3. Measurement of citrate levels in third‐instar larval fat bodies of wild type or dSeipin mutants grown on different foods, related to (A) (n = 3, fat bodies from 20 larvae were used for each repeat).
  4. Measurement of acetyl‐CoA levels in third‐instar larval fat bodies of wild type or dSeipin mutants grown on different foods, related to (A) (n = 3, fat bodies from 15 larvae were used for each repeat).
Data information: Error bars represent ±SEM (B–D) and statistical analyses were performed with one‐way ANOVA with a post‐Tukey's multiple comparison test (B). ***P < 0.001.