. 2018 Jun 23;6(4):405–427. doi: 10.1016/j.jcmgh.2018.06.006

Table 3.

Serum Iron, TIBC, and TF Saturation

Analysis	Genotype
Analysis	WT	Heph^-/-	Cp^-/-	Heph^-/-Cp^-/-	Fe deficient WT
Serum iron (μg/dL)	120 ± 29^a	85 ± 13^b	74 ± 16^b	13 ± 5^c	30
TIBC (μg/dL)	379 ± 39^a	462 ± 47^b	413 ± 27^a,b	874 ± 60^c	527
TF saturation^∗ (%)	30.3 ± 6.5^a	17.6 ± 1.0^b	18.0 ± 4.0^b	1.5 ± 0.5^c	5.7

NOTE. Serum iron, TIBC, and TF saturation as measured by ferrozine colorimetric assay in 9- to 11-week-old male littermates fed a chow diet and WT mice fed an iron-deficient diet for 6 weeks since weaning (Fe deficient WT). Mean ± SD; if no SD, only 1 animal was tested. Results for each analysis were examined by Welch t test and corrected for multiple comparisons with FDR. For each analysis, groups that share at least 1 superscript letter are not significantly different (FDR-adjusted P value >.05). Fe deficient WT mice were not included in the statistical analyses. N = 7 WT, 7 Heph^-/-, 14 Cp^-/-, and 10 Heph^-/-Cp^-/- mice. For TIBC and TF saturation, N = 6 WT, 6 Heph^-/-, 13 Cp^-/-, and 10 Heph^-/-Cp^-/- mice.

^∗

TF saturation assumes any iron detected in this assay is bound to TF in vivo.