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. 2018 Sep 1;32(17-18):1226–1241. doi: 10.1101/gad.314724.118

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© 2018 Zinoviev et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Purification of GTPBP1 and GTPBP2. (A) Domain organization of full-length and truncated GTPBP1 and GTPBP2. (B) Corresponding purified recombinant proteins, analyzed by SDS-PAGE. (C,D) The presence of GTPBP1 (C) and GTPBP2 (D) in RRL, Krebs II, and HeLa cell extracts, analyzed by SDS-PAGE followed by SimplyBlue staining (left panels) or Western blotting (right panels). (E, left panel) The scheme of GTPBP1 purification from RRL. (Right panel) Purified native and His-tagged recombinant GTPBP1 resolved by SDS-PAGE. Recombinant GTPBP1 migrates more slowly than the native factor due to the presence of the N-terminal His₆ tag.