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The GTPase activity of GTPBP1. (A–D) GTP hydrolysis by different forms of GTPBP1 in the presence or absence of various combinations of 80S ribosomes, ECs, and aa-tRNAs, assayed by thin-layer chromatography (TLC) and autoradiography. (A) The efficiency of GTP hydrolysis was quantified by phosphorimager and normalized to the condition with the highest GTP hydrolysis.
