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. 2018 Sep 1;32(17-18):1155–1160. doi: 10.1101/gad.313601.118

Figure 4.

Figure 4.

miR6026 targets slDCL2a/b/d transcripts and triggers secondary sRNA production. (A) Diagram of miR6026 target mimic. (B, top to bottom) Semiquantitative RT–PCR of miR6026 mimic RNA in wild-type and different mimicry lines with slActin7 as control. Northern blots of miR6026 with U6 as a loading control. (C) Three-week-old plants of wild type and one weak mimic line (L7) and two strong mimic lines of miR6026 (L2 and L6). (D) Quantitative RT–PCR (qRT–PCR) of slDCL2a and slDCL2b in wild-type and mimicry lines. (**) P < 0.01; (***) P < 0.001; (****) P < 0.0001. (E) RPM plot of total reads mapped to potential target transcripts of miR6026 in a weak mimic line (L7) and a strong mimic line (L6).