Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 1;32(17-18):1175–1187. doi: 10.1101/gad.311852.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 D'Amico et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — Loss of STAT3 accelerates KRAS-induced lung cell tumorigenesis. (A) Western blot analysis of nuclear and cytoplasmic extracts from control and STAT3 knockout KRAS^G12D p53 knockout lung epithelial cells (left panel) and cells transduced with wild-type or mutant STAT3 alleles (middle and right panels). (B) Growth and viability of KRAS^G12D p53 knockout lung epithelial cells in 2D monolayer (left panel) and 3D nonadherent (right panel) cultures. Relative cumulative cell numbers are shown in cultures for 1 wk. Values correspond to average and SD. Asterisks indicate a statistically significant difference compared with wild-type controls. (C) Subcutaneous tumor formation in nude mice by KRAS^G12D p53 knockout lung epithelial cells and mutant STAT3 derivatives (10⁴ cells per injection site). n ≥ 4 for each cell type. (D,E) H&E and IHC (KRT17/19, ΔNP63, and CD45) staining of tumors arising from KRAS^G12D p53 knockout lung epithelial cells shown in C. Representative images are shown. Bar, 200 µm.