Table 1.

PCR/RFLP conditions used to identify the sickle cell and G6PD genetic variants of individuals in the present study.

Mutations types	Codons	Primer oligonucleotide sequence (5′–3′)	Amplicon size (bp)	RFLP pattern (fragment size in bp)
				RE	Wild-type	Mutant
(Val → Met)	68	GTG GCT GTT CCG GGA	109	Hin1 II	109	63, 46
		TGG CCT TCT G
		CTT GAA GAA GGG CTC
		ACT CTG TTT G
(Glu → Val)	6	ACA CAA CTG TGT TCA	110	Bsu361 I	54, 56	110
		CTA GC
		CAA CTT CAT CCA CGT
		TCA CC

RFLP: Restriction Fragments Length Polymorphisms; RE: Restriction Enzyme; bp: base pairs.

Val: Valine; Met: Methionine; Glu: Glutamic acid.