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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Gastroenterology. 2018 May 18;155(3):865–879.e12. doi: 10.1053/j.gastro.2018.05.027

Figure 1. Gao-binge induces autophagic flux in mouse livers.

Figure 1

Male GFP-LC3 transgenic mice were treated with Gao-binge model. One dose of Leupeptin (40 mg/kg, i.p) was given to mice prior to the gavage. Mice were sacrificed 8 hours later after the gavage. (A) Representative confocal microscopy images from liver cryosections are shown. GFP-LC3 puncta per cell in each group were quantified (n=3-4). Ctrl: control diet+maltose gavage; EtOH: ethanol diet+ ethanol gavage. Data are means ± SE. More than 60 cells were counted in each mouse. Scale Bar: 10 μm. (B) Total liver lysates were subjected to western blot analysis. SE: short exposure; LE: long exposure. (C & D) Densitometry analysis of (B). The levels of p62 and LC3-II were normalized to the loading control (β-Actin). Data represent means ± SE (n= 3-4). *p<0.05; one-way ANOVA analysis.