Figure 5. Knockdown of hepatic TFEB exacerbates Gao-binge-induced liver injury and steatosis.
Male C57BL/6J WT mice were injected with Ad-shRNA negative and Ad-shRNA TFEB (1×109 PFU/mouse via tail vein) followed by Gao-binge model. (A) Total liver lysates were subjected to western blot analysis. (B) Hepatic mRNA was extracted followed by qPCR (n=4-6). Data are means ± SE. *p<0.05; Student t test. (C) Serum ALT levels and (D) hepatic TG levels were quantified. Data are means ± SE (n=4-6). * p<0.05; One-way ANOVA analysis. (E) Representative images of H& E staining are shown.