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. 2018 Sep;155(3):771–783.e3. doi: 10.1053/j.gastro.2018.05.050

Figure 5.

Figure 5

Dysregulated MIR194 expression drove cell proliferation in vitro through MIR194-GRHL3-PTEN axis. (A) Genomic alignment of GRHL3 mRNA 3′ UTR with MIR194. Normal esophageal cell line NES were transfected with either MIR194 plasmid (B) or anti-MIR194 (C). Relative expression of MIR194 (i), GRHL3 (ii), and PTEN (iii) in NES cell line was examined by qPCR Error bars show standard deviation of the mean: *P < .05; **P < .01. (D) Cell growth was determined by trypan blue exclusion assays every 24 hours for a period of 96 hours after MIR194 transfection relative to vector control. Error bars show standard deviation of the mean: *P < .05. (E) Diagram shows MIR194-GRHL3-PTEN cascade network.