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. 2018 Sep 3;8:13145. doi: 10.1038/s41598-018-31371-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FleQ is a global transcriptional regulator that can act both as an activator and as repressor in P. fluorescens F113 and P. putida KT2440. FleQ binding sites distribution along the P. fluorescens F113 (a) and P. putida KT2440 genomes (b). Fold enrichment value for each of the peaks after peak calling with MACS 1.4 is represented against the coordinates in which these accumulations are located. Red line marks the five-fold enrichment threshold fixed in the analysis. Venn diagram representation of the genes predicted to be in FleQ_F113 and FleQ_KT2440 regulons (c). Pie chart depicting the functional classification of FleQ-regulated genes shared by P. fluorescens F113 and P. putida KT2440. Percentage of the 41 genes shared by both species in each functional category according to Gene Ontology database is represented (d). Genes included in these graphs are listed in Table 1. Gene expression analysis of putative FleQ-regulated genes by RT-qPCR assays in P. fluorescens F113 (e) and P. putida KT2440 (f). Expression level in the wild-type strain was considered 1 for each of the tested genes. Fold variation for each gene was determined by the 2^−ΔΔCT method. RNA was extracted after growth in SA medium to an O.D.600 ≈ 0.8. The asterisks denote statistically significant differences (**P < 0.01, ***P < 0.001) found with t-test for independent samples and Bonferroni-Dune method.