Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 3;8:13134. doi: 10.1038/s41598-018-31372-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Maintenance of SRC-2 levels is required for EC cell accumulation and anchorage-independence. (A) Confirmation of siRNA-mediated knockdown of SRC-1, -2, and -3 by Western blot in EC cell lines. Uncropped images are provided in Supplemental Fig. S1. (B) Downregulation of SRC-2 but not -1 and -3 leads to reduction of cell colony formation by 3 EC cell lines (presented as quantification of extracted crystal violet used to stain formed colonies). (C) DNA content-based proliferation assay of Ishikawa cell line after knockdowns of SRCs demonstrates reduction of cell accumulation when SRC-2 levels are downregulated. (D) Examples of colonies formed by Ishikawa cells in soft agar after transfection with SRC-1, -2, or -3 siRNAs. A colony has been indicated with a white arrowhead. (E) Count of colonies formed in soft agar assay as shown in (D).