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. 2018 Sep 3;8:13134. doi: 10.1038/s41598-018-31372-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of RPIA is required for EC cell proliferation. (A) Quantification of colony formation assays performed in EC cell lines – Ishikawa, HEC-1-A, and AN3CA – after control, SRC-2, and RPIA siRNA-mediated knockdown. (B) DNA content-based proliferation assay performed with Ishikawa cells after control, SRC-2, and RPIA downregulation. (C) Examples of scatterplots representing measurements of propidium iodide and EdU incorporation of Ishikawa cells transfected with control, SRC-2, and RPIA siRNAs. (D) Quantification of hypo- and hyperploid cell populations and cells in G1, S, and G2 phases of the cell cycle evaluated by quantification of PI and EdU intensity as presented in (D).