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. 2018 Sep 3;8:13044. doi: 10.1038/s41598-018-31033-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alpha diversity and LEfSe analysis of fecal contents from sham and obstruction rats at genera level. Alpha diversity at genera level is represented in terms of Shannon diversity index and statistical significance was calculated using Mann-Whitney test and p < 0.05 is considered significant (A). LEfSe analysis for effect size measurement of differentially abundant genera in OB compared to sham rats is depicted in (B). Genera enriched in sham are represented in positive LDA score (green) and genera enriched in obstruction condition are represented in negative LDA score (red).