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. 2018 Sep 3;8:13125. doi: 10.1038/s41598-018-31433-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Eve expression in the developing embryo. Embryos expressing dCas9-VPR under the control of αTubulin-84b-GAL4 driver in the absence (left panels) and presence (right panels) of eve-sgRNA. (A) Primary antibody directed against Eve shows the canonical 7 striped band in a stage 6–7 embryo as well as (B) ectopic Eve protein throughout the embryo when eve-sgRNA is present. (C,D) DAPI staining of nuclei in stage 14–15 embryos. At stage 14–15 (E) wild-type expression pattern of Eve seen in anal pad and mesoderm/heart muscle and (F) ectopic expression of Eve is seen throughout the embryo when eve-sgRNA is present. (G,H) FLAG antibody indicates expression of N-terminal FLAG-tagged dCas9-VPR (Supplementary Fig. 1B) throughout the embryo. (I,J) FLAG, EVE composite micrographs. Scale bar: 100 µm. Anterior to left, posterior to right in all images.